CRISPR/Cas9 screening using unique molecular identifiers
نویسندگان
چکیده
منابع مشابه
CRISPR/Cas9 screening using unique molecular identifiers
Loss-of-function screening by CRISPR/Cas9 gene knockout with pooled, lentiviral guide libraries is a widely applicable method for systematic identification of genes contributing to diverse cellular phenotypes. Here, Random Sequence Labels (RSLs) are incorporated into the guide library, which act as unique molecular identifiers (UMIs) to allow massively parallel lineage tracing and lineage dropo...
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ژورنال
عنوان ژورنال: Molecular Systems Biology
سال: 2017
ISSN: 1744-4292,1744-4292
DOI: 10.15252/msb.20177834